Product Name: SEPHADEX G-50
Synonyms: PDXTM G.F.;Sephadex(R) G-50 Fine;PDX GF 50 (100-300);PDX GF 50 (50-150);SEPHADEX G-50;SEPHADEX G-50 COARSE;SEPHADEX G-50 FINE;Sephadex G-50, Fine beads
CAS: 9048-71-9
MF: NULL
MW: 0
Product Categories: DNA&RNA Purification;Molecular Biology;Reagents;Gel Filtration Media;Sephadex G-50;Gel Filtration Chromatography;Gel Filtration MediaGel Filtration Media;Protein Chromatography;Sephadex
Uses The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.
lower limit upper limit
dextran gels
Sephadex G-50 1.5 30
Sephadex G-75 3 80
Sephadex G-100 4 150
Sephadex G-150 5 300
Sephadex G-200 5 600
polyacrylamide gels
Bio-Gel P-10 1.5 20
Bio-Gel P-30 2.5 40
Bio-Gel P-60 3 60
Bio-Gel P-100 5 100
Bio-Gel P-150 15 150
BioGel P-200 30 200
Bio-Gel P-300 60 400
dextran-polyacrylamide gels
Sephacryl S-200 5 250
Sephacryl S-300 10 1500
Sephacryl S-400 20 8000
agarose gels
Sepharose 6B 10 4000
Sepharose 4B 60 20,000
Sepharose 2B 70 40,000
Bio-Gel A-0.5 10 500
Bio-Gel A-1.5 10 1500
Bio-Gel A-5 10 5000
Bio-Gel A-15 40 15,000
Bio-Gel A-50 100 50,000